Aprendizado e Memória de Trabalho em Camundongos sob Diferentes Condições de Iluminação
DOI:
https://doi.org/10.34024/rnc.2013.v21.8158Palavras-chave:
Ritmos Circadianos, Memória de Trabalho, CogniçãoResumo
Objetivo. Investigar o efeito de diferentes ciclos claro/escuro e da intensidade luminosa durante testes comportamentais de aprendizagem e memória de trabalho em camundongos suíços. Método. Cinquenta e sete camundongos foram mantidos em um biotério nas seguintes condições: 12:12h de ciclo claro/escuro (LD), luz constante (LL), ou escuridão constante (DD). Os animais foram, então, testados no labirinto de Lashley e no teste de reconhecimento de objetos sob 500 ou 0 lux de iluminação, resultando em seis grupos (LD-500, LD-0, LL-500, LL-0, DD-500 e DD-0). Resultados. Não houve diferenças significativas entre as condições de luz ou escuridão, ou na iluminação entre 500 e 0 lux nos testes. Animais mantidos em constante escuridão e testados a 0 lux (DD-0) tiveram a aprendizagem e a memória de trabalho prejudicadas, como demonstrado pelo aprendizado lento no labirinto Lashley, e ausência do reconhecimento de objetos na tarefa de reconhecimento de objetos. Conclusão. O escuro contínuo durante todo o experimento afetou o aprendizado e a memória de trabalho em camundongos.
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Publicado: 2013-09-30
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