Efeitos do extrato de lúpulo num modelo animal de fibromialgia induzido por de reserpina
DOI:
https://doi.org/10.34024/rnc.2023.v31.14965Palavras-chave:
Fibromialgia, Depressão, Ansiedade, Modelos Animais, Lúpulo, ReserpinaResumo
Introdução. O diagnóstico da fibromialgia vem sendo frequentemente atualizado incluindo sinais característicos do binômio depressão/ansiedade. Objetivo. Estabelecer as características de estados de depressão/ansiedade em um modelo animal de fibromialgia induzido por injeções repetidas de reserpina em ratos Wistar sob o efeito do tratamento com extrato hidroalcoólico de lúpulo. Método. Após injeções de salina, reserpina e extrato hidroalcoólico de lúpulo, ratas Wistar foram submetidas aos testes do campo aberto (TCA), labirinto em cruz elevado (LCE) e teste do nado forçado (TNF). Resultados. Após o tratamento com extrato de lúpulo não foi possível observar diferenças no TCA e no LCE se comparado à reserpina. Por outro lado, o extrato hidroalcoólico de lúpulo foi capaz de reverter as alterações comportamentais depressivas produzidas pela reserpina no TNF. Conclusão. Esses dados reforçam o efeito depressivo e ansiogênico característico do modelo de fibromialgia induzido por injeções repetidas de reserpina e sugerem, também, potencial efeito ansiolítico do extrato de lúpulo.
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Sarzi-Puttini P, Giorgi V, Marotto D, Atzeni F. Fibromyalgia: an update on clinical characteristics, aetiopathogenesis and treatment. Nat. Rev Rheumatol 2020;16:645-60. https://doi.org/10.1038/s41584-020-00506-w
Pereira CN, Nunes BF, André, ES. Investigação das correlações existentes entre o ciclo vigília-sono e a dor num modelo experimental de fibromialgia. Biotemas 2020;33:99-108. https://doi.org/10.5007/2175-7925.2020.e74455
Siracusa R, Fusco R, Cuzzocrea S. Fibromyalgia: Pathogenesis, mechanisms, diagnosis, and treatment options update. Inter J Mol Sci 2021;22:3891. https://doi.org/10.3390/ijms22083891
Sturge-Jacobs M. The experience of living with fibromyalgia: confronting an invisible disability. Res Nursing Pract 2002;16:19-28. https://doi.org/10.1891/rtnp.16.1.19.52994
Aguglia A, Salvi V, Maina G, Rossetto I, Aguglia E. Fibromyalgia syndrome and depressive symptoms: Comorbidity and clinical correlates. J Affect Dis 2011;128:262-6. https://doi.org/10.1016/J.JAD.2010.07.004
Bradley LA. Psychiatric comorbidity in fibromyalgia. Curr Pain Headache Rep 2005;9:79-86. https://doi.org/10.1007/s11916-005-0042-3
Fuller-Thomson E, Nimigon-Young J, Brennenstuhl S. Individuals with fibromyalgia and depression: findings from a nationally representative Canadian survey. Rheumatol Int 2012;32:853-62. https://doi.org/10.1007/s00296-010-1713-x
Gracely RH, Ceko M, Bushnell MC. Fibromyalgia and depression. Pain Res Treat 2012;2012:486590. https://doi.org/10.1155/2012/486590
Thieme K, Turk DC, Flor H. Comorbid depression and anxiety in fibromyalgia syndrome: relationship to somatic and psychosocial variables. Psychosom Med 2004;66:837-44. https://doi.org/10.1097/01.psy.0000146329.63158.40
Russell IJ. The promise of substance P inhibitors in fibromyalgia. Rheu Dis Clin North Am 2002;28:329-42. https://doi.org/10.1016/S0889-857X(01)00006-0
Sluka KA. Is it possible to develop an animal model of fibromyalgia? Pain 2009;146:3-4. https://doi.org/10.1016/j.pain.2009.07.032
DeSantana JM, Cruz KM, Sluka KA. Animal models of fibromyalgia. Arthritis Res Ther 2013;15:222. https://doi.org/10.1186/ar4402
De la Luz-Cuellar YE, Rodríguez-Palma EJ, Franco-Enzástiga Ú, Déciga-Campos M, Mercado F, Granados-Soto V. Spinal dopaminergic D1 and D5 receptors contribute to reserpine-induced fibromyalgia-like pain in rats. Brain Res 2023;1799:148167. https://doi.org/10.1016/j.brainres.2022.148167
Adrien J. Neurobiological bases for the relation between sleep and depression. Sleep Med Rev 2002;6:341-51. https://doi.org/10.1053/smrv.2001.0200
Jacobsen JP, Medvedev IO, Caron MG. The 5-HT deficiency theory of depression: perspectives from a naturalistic 5-HT deficiency model, the tryptophan hydroxylase 2Arg439His knockin mouse. Philos Trans R Soc Lond B Biol Sci 2012;367:2444-59. https://doi.org/10.1098/rstb.2012.0109
Lacerda-Pinheiro SF, Pinheiro Junior RF, Lima MAP, Silva CGL, Santos MSV, Teixeira Júnior AG, et al. Are there depression and anxiety genetic markers and mutations? A systematic review. J Affect Disord 2014;168:387-98. https://doi.org/10.1016/j.jad.2014.07.016
Borchers AT, Gershwin ME. Fibromyalgia: A Critical and Comprehensive Review. Clin Rev Allergy Immunol 2015;49:100-51. https://doi.org/10.1007/s12016-015-8509-4
Nagakura Y, Oe T, Aoki T, Matsuoka N. Biogenic amine depletion causes chronic muscular pain and tactile allodynia accompanied by depression: A putative animal model of fibromyalgia. Pain 2009;146:26-33. https://doi.org/10.1016/j.pain.2009.05.024
Schuldiner S, Shirvan A, Linial M. Vesicular neurotransmitter transporters: from bacteria to humans. Physiol Rev 1995;75:369-92. https://doi.org/10.1152/physrev.1995.75.2.369
Stitzel RE, Craig C. Farmacologia Moderna com Aplicações Clínicas. 3a.ed. Rio de Janeiro: Guanabara-Koogan; 2005; 832p.
Beppler LM. Efeito do extrato hidroalcóolico da planta Polygala paniculata no modelo de fibromialgia induzido pela reserpina em camundongos: avaliação sensorial e emocional (Dissertação). Florianópolis: Universidade Federal de Santa Catarina; 2019. https://repositorio.ufsc.br/handle/123456789/198618
Lobay D. Rauwolfia in the treatment of hypertension. Integ Med 2015;14:40-6. https://europepmc.org/article/MED/26770146#free-full-text
Chadwick LR, Pauli GF, Farnsworth NR. The pharmacognosy of Humulus lupulus L. (hops) with an emphasis on estrogenic properties. Phytomedicine 2006;13:119-31. https://doi.org/10.1016/j.phymed.2004.07.006
Zanoli P, Zavatti M. Pharmacognostic and pharmacological profile of Humulus lupulus L. J Ethnopharmacol 2008;116:383-96. https://doi.org/10.1016/j.jep.2008.01.011
Carlini EA, Mendes FR. Protocolos em Psicofarmacologia Comportamental: um Guia para a Pesquisa de Drogas com Ação sobre o SNC, com Ênfase em Plantas Medicinais. São Paulo: FAP-UNIFESP; 2012.
Pellow S, Chopin P, File SE, Briley M. Validation of open:closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 1985;14:149-67. https://doi.org/10.1016/0165-0270(85)90031-7
Treit D, Menard J, Royan C. Anxiogenic stimuli in the elevated plus-maze. Pharmacol Biochem Behav 1993;44:463-9. https://doi.org/10.1016/0091-3057(93)90492-c
Slattery DA, Cryan JF. Using the rat forced swim test to assess antidepressant-like activity in rodents. Nat Protoc 2012;3:1009-14. https://doi.org/10.1038/nprot.2012.044
Holmes A. Targeted gene mutation approaches to the study of anxiety-like behavior in mice. Neurosci Biobehav Rev 2001;25:261-73. https://doi.org/10.1016/s0149-7634(01)00012-4
Freitas CM, Busanello A, Schaffer LF, Peroza LR, Krum BN, Leal CQ, et al. Behavioral and neurochemical effects induced by reserpine in mice. Psychopharmacology (Berl) 2016;233:457-67. https://doi.org/10.1007/s00213-015-4118-4
Blasco-Serra A, Escrihuela-Vidal E, González-Soler EM, Martínez-Expósito E, Blasco-Ausina MC, Martínez-Bellver S, et al. Depressive-like symptoms in a reserpine-induced model of fibromyalgia in rats. Phys Behav 2015; 151:456–462. https://doi.org/10.1016/j.physbeh.2015.07.033
Shishegar R, Rezaie A, Nazeri M. Study of sedation, pre-anesthetic and antianxiety effects of hop (Humulus lupulus L.) Extract compared with diazepam in rats. J Anim Vet Adv 2012;11:2570-5. https://doi.org/10.3923/javaa.2012.2570.2575
Zanoli P, Rivasi M, Zavatti M, Brusiani F, Baraldi M. New insight in the neuropharmacological activity of Humulus lupulus L. J Ethnopharmacol 2005;102:102-6. https://doi.org/10.1016/j.jep.2005.05.040
Heinrich M, Barnes J, Gibbons S, Williamson EM (Eds.). Fundamentals of Pharmacognosy and Phytotherapy. Edinburgh: Elsevier, Churchill Livingstone; 2012; p.243.
Lee KM, Jung JS, Song DK, Krauter M, Kim YH. Effects of humulus lupulus extract on the central nervous system in mice. Planta Med 199;359(Suppl.7):A691. https://doi.org/10.1055/s-2006-959980
Bravo L, Cabo J, Fraile A, Jimenez J, Villar A. Estudio farmacodinamico del lupulo (Humulus lupulus L.) – acción tranquilizante. Boll Chim Farmac 1974;113:310-5. https://doi.org/ 10.1556/APhysiol.99.2012.2.6
Charney DS, Heninger GR, Sternberg DE. Serotonin function and mechanism of action of antidepressant treatment. Effects of amitriptyline and desipramine. Arch Gen Psychiatry 1984;41:359-65. https://doi.org/10.1001/archpsyc.1984.01790150049008
Zanoli P, Zavatti M, Rivasi M, Brusiani F, Losi G, Puia G, et al. Evidence that the beta-acids fraction of hops reduces central GABAergic neurotransmission. J Ethnopharmacol 2007;109:87-92. https://doi.org/10.1016/j.jep.2006.07.008
Kyrou I, Christou A, Panagiotakos D, Stefanaki C, Skenderi K, Katsana K, et al. Effects of a hops (Humulus lupulus L.) dry extract supplement on self-reported depression, anxiety and stress levels in apparently healthy young adults: a randomized, placebo-controlled, double-blind, crossover pilot study. Hormones (Athens) 2017;16:171-80. https://doi.org/10.14310/horm.2002.1738
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Copyright (c) 2023 Maria Eduarda Rodrigues, Eduardo Peretti dos Santos, Cláudio Laurentino Guimarães, Edison Sanfelice André
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Aceito: 2023-09-13
Publicado: 2023-09-20
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